Research Article

Evaluation of Salivary Superoxide Dismutase Levels in Oral Submucous Fibrosis – A Cross Sectional Study

Kavita Nagar
* Corresponding author
G.P. Sujatha
Ashok Lingappa
DOI icon 10.24018/ejmed.2021.3.3.864

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Submitted 2021-05-05
Published 2021-05-27

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Article Main Content

Background: Oral Submucous Fibrosis (OSMF) is a potentially malignant disorder with areca nut being the main etiological agent. Increased risk of malignant potential of OSMF necessitates the early diagnosis of the condition. Superoxide dismutase (SOD) scavenges oxygenated free radicals and can be used as a potential biomarker for oral cancer detection.

Objectives: The present study was conducted to estimate and compare salivary SOD levels in patients with OSMF and healthy controls and to compare its levels in different clinical and functional stages of OSMF.

Methodology: The study group (Group 1) included 30 patients of OSMF, and the control group (Group 2) was formed by age and sex-matched healthy individuals. Salivary SOD levels were determined by Nitro Blue Tetrazolium (NBT) chloride method. The levels of salivary SOD were compared between the two groups and the data thus obtained was subjected to statistical analysis using independent sample t-test and one way Analysis of variance (ANOVA) test.

Results: The mean salivary SOD in the study group was 0.583 IU/mL and 0.864 IU/mL in control group and the difference was found to be statistically significant. Thus, the patients with OSMF had significantly lower levels of salivary SOD compared to the controls.

Conclusion: The decrease in the salivary SOD levels in patients with OSMF suggests the role of oxidative stress in the pathogenesis of OSMF and initiation and progression of carcinogenesis. Thus, it can be used as a prognostic marker for early detection of carcinoma in patients with OSMF and thereby, improving the quality of life patients.

Keywords: Antioxidant, Oral submucous fibrosis, Superoxide dismutase

References

  1. Prabhu RV, Prabhu V, Chatra L, Shenai P, Suvarna N, Dandekeri S. Areca nut and its role in oral submucous fibrosis. Journal of Clinical and Experimental Dentistry. 2014;6(5):e569-e575.
     Google Scholar
  2. Pindborg JJ, Sirsat SM. Oral submucous fibrosis. Oral Surg Oral Med Oral Pathol. 1966;22:764–79.
     Google Scholar
  3. Arakeri G, Brennan PA. Oral submucous fibrosis: an overview of the aetiology, pathogenesis, classification, and principles of management. Br J Oral Maxillofac Surg. 2013 Oct;51(7):587-93.
     Google Scholar
  4. More CB, Das S, Patel H, Adalja C, Kamatchi V, Venkatesh R. Proposed clinical classification for oral submucous fibrosis. Oral Oncol. 2012;48(3):200-2.
     Google Scholar
  5. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans, World Health Organization, International Agency for Research on Cancer. Betel-quid and areca-nut chewing and some areca-nut-derived nitrosamines. 2004.
     Google Scholar
  6. Paymaster JC. Cancer of the buccal mucosa; a clinical study of 650 casesin Indian patients. Cancer 1956;9:431–5.
     Google Scholar
  7. Pindborg JJ, Murti PR, Bhonsle RB, Gupta PC, Daftary DK, Metha F. Oral submucous fibrosis as a precancerous condition. Scand J Dent Res 1984;92:224–9.
     Google Scholar
  8. Murti PR, Bhonsle RB, Pindborg JJ, Daftary DK, Gupta PC, Mehta FS. Malignant transformation rate in oral submucous fibrosis over a 17-year period. Community Dent Oral Epidemiol. 1985 Dec;13(6):340-1.
     Google Scholar
  9. Bathi RJ, Rao R, Mutalik S. GST null phenotype and antioxidants; risk indicators for oral precancer and cancer. Indian J Dent Res 2009;20:298-302.
     Google Scholar
  10. Michelson AM. Toxicity of superoxide radical anions. In: Michelson AM, McCord JM, Fridovich. Superoxide and Superoxide Dismutase 1st ed. London, UK: Academic Press;1977.
     Google Scholar
  11. Shetty SR, Babu SG, Kumari S, Karikal A, Shetty P, Hegde S. Salivary superoxide dismutase levels in oral leukoplakia and oral squamous cell carcinoma; a clinicopathological study. Oxid Antioxid Med Sci. 2013;2(1):69-71.
     Google Scholar
  12. Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971;44(1):276-87.
     Google Scholar
  13. Patel BP, Rawal UM, Rawal RM, Shukla SN, Patel PS. Tobacco, antioxidant enzymes, oxidative stress, and genetic susceptibility in oral cancer. Am J Clin Oncol. 2008 Oct;31(5):454-9.
     Google Scholar
  14. Madalli VB, Basavaraddi SM, Burde K, Horatti P. Saliva-A diagnostic tool. IOSR J Dent Med Sci. 2013 Nov;11:96-99.
     Google Scholar
  15. Metkari SB, Tupkari J V, Barpande S R. An estimation of serum malondialdehyde, superoxide dismutase and vitamin A in oral submucous fibrosis and its clinicopathologic correlation. J Oral Maxillofac Pathol 2007;11:23-7.
     Google Scholar
  16. Gurudath S, Naik RM, Ganapathy K S, Guruprasad Y, Sujatha D, Pai A. Superoxide dismutase and glutathione peroxidase in oral submucous fibrosis, oral leukoplakia, and oral cancer: A comparative study. J Orofac Sci 2012;4:114-9.
     Google Scholar
  17. Bale R, Kattappagari KK, Vidya D, Vuddandi S, Gummalla C, Baddam VR. Oral submucous fibrosis: A quantitative assessment of serum malondialdehyde, superoxide dismutase and correlation with clinical staging. J Oral Maxillofac Pathol 2017;21:41-5.
     Google Scholar
  18. Sadaksharam J. Significance of Serum Nitric Oxide and Superoxide Dismutase in Oral Submucous Fibrosis and Squamous Cell Carcinoma: A Comparative Study. Contemporary Clinical Dentistry. 2018;9(2):283-288.
     Google Scholar